ABSTRACT
Objective: To explore the effect of age on Qingkailing Granules disposition by comparing the pharmacokinetics of geniposide and baicalin in juvenile and adult rats. Methods: A simple and rapid LC-MS/MS method was developed and validated to simultaneously determine geniposide and baicalin in rat plasma after a simple protein precipitation. The analytes were separated on an Agilent ZORBAX Extend-C18 column. The mobile phase consisted of acetonitrile and water with 0.1% (volume percent) formic acid at a flow rate of 0.6 mL/min. The ionization was conducted using an ESI source in negative ion mode. Multiple reaction monitoring was used for quantification at transitions of m/z 445.0 → m/z 268.9 for baicalin, m/z 433.2 → m/z 225.0 for geniposide, m/z 431.0 → m/z 341.0 for vitexin (IS). Juvenile and adult rats were administrated Qingkailing Granules (3 g/kg) orally. Plasma concentrations of baicalin and geniposide were determined by LC-MS/MS. Results: The linear ranges of the analytes were 1–1000 ng/mL for baicalin and 2–2000 ng/mL for geniposide. The method was successfully applied to compare the pharmacokinetics of the analytes between juvenile and adult rats after oral administration of Qingkailing Granules. AUC was bigger in adult rats, while t1/2 was longer in juvenile rats. Conclusion: These results suggested that the absorption and elimination of baicalin and geniposide in juvenile rats was lower than that in adult rats. Additional attention should be paid to the pharmacokinetic difference when Qingkailing Granules were used in children.
ABSTRACT
AIM: To establish a method for determining baicalin, jasminoidin, cholalic acid and hyodeoxycholic acid in Qingkailing Granules(chololic acid, hyodeoxycholic acid, baicalin, Fructus Gardeniae, Cornu Bubali, Flos Longicerae Japonicae, Radix lsatidis, Concha Margaritifera). METHODS: Part 1 (determination of baicalin and jasminoidin):The HPLC method was carried out on kromasil~(TM)C_(18) column(4.6 mm×150 mm,5 μm) with acetonitrile-water(10: 90)as mobile phase, gradient elution; the UV detection wavelength was at 238 nm. Part 2 (determination of cholalic acid and hyodeoxycholic acid):The HPLC method was carried out on kromasil~(TM)C_(18) column(4.6 mm×150 mm,5 μm) with acetonitrile-water-phosphonic acid(35:65:0.1) as mobile phase,gradient elution;the UV detection wavelength was at 192 nm. RESULTS: The average recoveries were 99.30% with RSD of 0.2% for baicalin; 99.50% with RSD of 0.4% for jasminoidin; 99.04% with RSD of 0.2% for hyodeoxycholic acid; 99.06% with RSD of 0.4% for cholalic acid; respectively. CONCLUSION: The assay demonstrats that the method is simple,it has the adequate accuracy and selectivity to quantify the four active components in Qingkailing Granules.
ABSTRACT
AIM: To establish a method for determining baicalin,jasminoidin,cholalic acid and hyodeoxycho-lic acid in Qingkailing Granules(chololic acid,hyodeoxycholic acid,baicalin,Fructus Gardeniae,Cornu Bubali, Flos Longicerae Japonicae,Radix Isatidis,Concha Margaritifera). METHODS: Part 1(determination of baicalin and jasminoidin):The HPLC method was carried out on kromasilTMC_18 column(4.6 mm?150 mm,5 ?m) with acetonitrile-water(10∶90)as mobile phase,gradient elution;the UV detection wavelength was at 238 nm.Part 2(determination of cholalic acid and hyodeoxycholic acid):The HPLC method was carried out on kromasilTMC_18 column(4.6 mm?150 mm,5 ?m) with acetonitrile-water-phosphonic acid(35∶65∶0.1) as mobile phase,gradient elution;the UV detection wavelength was at 192 nm. RESULTS: The average recoveries were 99.30% with RSD of 0.2% for baicalin;99.50% with RSD of 0.4% for jasminoidin;99.04% with RSD of 0.2% for hyodeoxycholic acid;99.06% with RSD of 0.4% for cholalic acid;respectively.CONCLUSION: The assay demonstrats that the method is simple,it has the adequate accuracy and selectivity to quantify the four active components in Qingkailing Granules.